Protein crystallography resource at neutron research center for imaging proteins
The neutron diffraction station may provide critical data for pharmaceutical companies to develop new designer
drugs to combat debilitating diseases. It also may allow genetic researchers to understand better the events that lead
to activation of "genetic switches" that result in deformities or maladies. Genetic switches might also fight off illnesses
or trigger immune responses.
Understanding the structure of proteins and polymers is also key to understanding the messages encoded in human DNA.
At the facility, researchers place a sample of a material in the path of a beam of neutrons. Neutrons in the beam scatter
when they interact with the atoms in the sample. A detector behind the sample records how the neutrons were
scattered and renders a two-dimensional, black-and-white stippled image— a crude mandala of sorts. This diffraction
pattern is manipulated and then analyzed by a computer to reveal the 3-D structure of the sample. The computer
analysis accurately portrays the relative distances between the atoms that make up the structure, the lengths and angles
of the bonds between atoms in the structure and the position and location of each hydrogen atom in the structure.
The neutron-diffraction-imaging process is a little like making and interpreting an X-ray image. In fact, structural
biologists use X-ray diffraction to make images of organic molecules. But unlike X-ray diffraction, which is "blind" to
hydrogen, neutron diffraction yields accurate information about hydrogen atoms contained in a structure. Being able
to see and understand hydrogen atoms in a polymer, like DNA, or in a protein segment is key to understanding how
the protein or polymer functions. Hydrogen is key to many internal and external reactions of proteins with other
biological chemicals. Hydrogen atoms act like tiny switches to activate a chemical or to keep it in "standby" mode.
Because ordinary hydrogen can be substituted by deuterium—a stable isotope of hydrogen that has an extra neutron in
its nucleus—and because the neutron scattering data can distinguish between hydrogen and deuterium atoms, scientists
will be able to get a better idea of which hydrogen atoms are actually switches and which are simply chemical place-
holders in a molecule.
Moreover, because neutron diffraction can see hydrogen, researchers will be able to better understand the role that
water (two hydrogen atoms bound to an oxygen atom) plays in proteins and polymers. In some cases, water
molecules are simply a structural building block. In others, water arranges itself into a funnel configuration that
allows other chemicals to move from one area to another. Understanding the role of water in biological chemicals is a
key field of study for some Los Alamos researchers.
The Department of Energy's Office of Science is the single largest supporter of basic research in the physical sciences in the United States and is working to address some of the most pressing challenges of our time.