A Highly Sensitive Microsphere-Based Assay for Early Detection of Type I Diabetes (IMAGE)

World Scientific

A Highly Sensitive Microsphere-Based Assay for Early Detection of Type I Diabetes

Caption

This is a concept showing microsphere based detection of an Islet Cell Autoantibody (ICA) - Glutamic Acid Decarboxylase (GAD65) using Rolling Circle Amplification (RCA) on microspheres. Streptavidin-microspheres are conjugated with biotinylated-GAD65 followed by binding of GAD65Ab to microspheres. To these microspheres, biotin-GAD65 is added and coupled with bioyinylated DNA-primer via an avidin bridge. To the immobilized target primer, RCA circle is added followed by elongation using Phi 29 Polymerase and detection using SYBR Green II fluorescence.

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<i>Technology</i> journal

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