Fluo4-AM (IMAGE) Osaka University Caption Mouse olfactory epithelium-derived cells were labeled with Fluo4-AM (a), and applied to a 10-μm microchamber array chip equipped with a flow chamber, followed by brief centrifugation (b). Floating and untrapped cells were removed by circulating Ringer solution and stimulated with an odorant (c). OSNs expressing odorant-specific ORs showed transient fluorescence from the increase of intracellular Ca2+ concentration (d). Each activated cell was retrieved (e), transferred to the 96-well PCR plate (f), and subjected to single-cell RT-PCR. Credit Osaka University Usage Restrictions None License Licensed content Disclaimer: AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert system.