Graphic Schematic and Photograph of the Bi-Layer PharmaFlux Device (IMAGE)

Lehigh University

Graphic Schematic and Photograph of the Bi-Layer PharmaFlux Device

Caption

This is a graphical schematic (left) and photograph (center) of the bi-layer device showing the upper (marked with yellow lines) and lower (marked with red lines) channel separated by a semi-permeable membrane. The schematic on the right depicts the culture of a monolayer of endothelial cell on the semi-permeable membrane and the manner in which TNF-α treatment is performed from the lower channel. B, (i) Bright field image of the upper (marked with yellow lines) and lower (marked with red lines) channel defining the upstream, TNF-α activated and downstream sections, (ii) Fluorescently labeled F-actin cytoskeleton (FITC phalloidin) images of confluent BAOEC layer aligned to flow (12 dyne/cm2 FSS for 24 hrs). The cells are cultured on the semi-permeable membrane in the upper channel of the device. Arrow shows flow direction (Scale bar: 100 μm)

Credit

Yaling Liu

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