Manganese Blocks Intracellular Trafficking of Shiga Toxin (video) Carnegie Mellon University Share Print E-Mail Loading video... Caption In control cells, GPP130 cycles from the Golgi to endosomes and back. After internalization into endosomes, Shiga toxin binds GPP130 and is sorted into endosome membrane tubules that break off and deliver the toxin to the Golgi. From there it moves to the ER and then the cytoplasm causing cell death. In manganese treated cells, GPP130 moves to lysosomes and is degraded. After internalization into endosomes, Shiga toxin stays in the larger part of the endosome because of the absence of GPP130. As a consequence, Shiga toxin moves to lysosomes (the default pathway for internalized content) where it is degraded. Credit Carnegie Mellon University Usage Restrictions None Share Print E-Mail Disclaimer: AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert system.