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3-D Printed Materials Resemble Biological Tissues (8 of 9)

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Caption: Printing of extended droplet network: Time-lapse movie of an extended droplet network being printed in bulk lipid-in-oil solution. The interval between frames is 20 s. Each frame of the video was cropped around the constructed-network for ease of viewing, as the original recording focused on the stationary capillary-tip, whilst the oil-well was moved in relation to the tip during printing. The printed network consisted of 22 layers, with each layer based on a 9×9 square and comprised of two droplet-types. Blue and yellow droplets were made from a solution of 25 mM Tris•HCl, 1M KCl, 100 μM EDTA at pH 8.0 and contained 1 mM xylene cyanol FF and 10 mM orange G respectively. Droplets were ejected into a mixture of silicone oil AR20 and hexadecane (1:1 V/V) containing 0.2 mg mL-1 1,2-diphytanoyl-sn-glycero-3-phosphocholine. The tip-end diameter of the capillary filled with yellow solution is 150 μm. This video relates to a paper that appeared in 5 April, 2013, issue of Science, published by AAAS. The paper, by Gabriel Villar at the University of Oxford in Oxford, UK, and colleagues was titled, “A Tissue-Like Printed Material.”

Credit: [Video courtesy of Alexander Graham]

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