CLARITY provided this 3-D view showing a thick slice of a mouse brain's memory hub, or hippocampus. It reveals a few different types of cells: Projecting neurons (green), connecting interneurons (red), and layers of support cells, or glia (blue). Conventional 2-D methods require that brain tissue be thinly sliced, sacrificing the ability to analyze such intact components in relation to each other. CLARITY permits such typing of molecular and cellular components to be performed repeatedly in the same brain.
Karl Deisseroth, M.D., Ph.D., Stanford University