Oligomerization of WT and AW -VP40 in Live CHO Cells (image) Cell Press Share Print E-Mail Caption The number and brightness method (Ref) was used to analyze RICS data acquired with a laser scanning confocal microscope. Left column: Fluorescent intensity image of CHO cells transfected with EGFP (control), WT-VP40 tagged EGFP and AW-VP40-EGFP. Middle: Formation of oligomers were assessed by determining the brightness of fluorescently tagged proteins. Brightness vs intensity plots were done using SimFSC software. Selection boxes chosen depict monomer (red), Octamers (green) as well as molecules with sizes > 10mers . Right: Selection map of the respective cells showing sub cellular distribution oligomers. Scale bar = 3.2 µm Credit Biophysical Journal, Soni et al. Figure S2 Usage Restrictions Credit Required Share Print E-Mail Disclaimer: AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert system.