The schematic represents an
EM Affinity Grid (gray square) coated with adaptor molecules (red and dark blue) that anchor active rotavirus
DLPs (yellow) to the Affi nity Grid. Cryo-Electron Microscopy (EM) image of actively transcribing DLPs reveals
RNA strands (gray strands) emerging from the virus capsid. Three-dimensional image reconstructions of DLPs
(light blue) that actively produce RNA reveal strong density within the viral core. Diameter of each reconstruction
is ~80 nm.