Creating transgenic mice, while critical to biomedical research, is laborious and expensive, despite improvements since the advent of CRISPR-Cas9 gene editing. Now, UC Berkeley biologists have invented a technique that simplifies, improves and lowers the cost of generating knockout mice. They discovered that electroporation can move CRISPR-Cas9 molecules into mouse embryos with nearly 100 percent efficiency, much better than the success from microinjecting Cas9 mRNA and guide RNA. The gene-editing success is also higher.
- Journal of Biological Chemistry
- NIH/National Cancer Institute, NIH/National Institute of General Medical Sciences