Two studies by a team of researchers led by Luis E. Cuevas and Mohammed Yassin from the Liverpool School of Tropical Medicine and jointly coordinated with Andrew Ramsay at WHO-TDR Special Programme for Research and Training in Tropical Diseases are published in this week's PLoS Medicine. The studies have important implications for the ways in which diagnosis for the endemic infectious disease, tuberculosis (TB), can be done in poor countries. One study suggests that less sputum tests collected the same day of consultation are needed and the other suggests that a faster laboratory test can be used while maintaining the same level of accuracy for diagnosis. Both studies show that alternative, less labour-intensive tests that are more convenient for patients could be effectively used in poorer countries.
The researchers enrolled 6627 patients in Ethiopia, Nepal, Nigeria and Yemen who had had a cough for more than two weeks (a characteristic symptom of tuberculosis).
In the main trial the centers participating in the study were randomly assigned each week for a year to use different methods of sputum collection. The results suggest that a sputum collection scheme in which two samples are collected one hour apart followed by a morning specimen could identify as many smear-positive patients as the standard "spot-morning-spot" scheme in which patients provide an on-the-spot specimen during their initial consultation, a specimen collected at home the next morning, and another on-the-spot specimen when they bring their morning specimen to the clinic. The study also confirmed that examination of the first two specimens alone identifies most smear-positive patients, independently of which scheme is used.
The authors say: "The identification of the majority of smear-positive patients may require no more than one patient visit, and the scheme presented here has the potential to improve the diagnosis of pulmonary tuberculosis in Low and Middle-Income Countries. A single-visit diagnosis would represent a substantial opportunity to improve the delivery of TB services, particularly to the poor."
In the second study, which is a sub-study of the main trial, the researchers examined nearly 2,400 patients to show that a faster laboratory test, a variant form of smear microscopy—light emitting-diode fluorescence microscopy (LED-FM)— could identify more people with tuberculosis than the standard smear microscopy test (in which technicians use a stain called Ziehl Neelsen from a patient's sputum). However LED-FM might also lead to more people without tuberculosis being needlessly treated, as this test picks up more false positives, that is, people who don't have TB but who are incorrectly classified as test-positive for the disease.
The authors conclude: "This study has shown that LED-FM can play a key role in reaching the [World Health Organization] targets for TB detection, reducing laboratory workloads, and ensuring poor patients' access to TB diagnosis and prompt treatment."
Main study: "A Multi-Country Non-Inferiority Cluster Randomized Trial of Frontloaded Smear Microscopy for the Diagnosis of Pulmonary Tuberculosis"
Funding: This research was funded by the Bill & Melinda Gates Foundation and the United States Agency for International Development through grants awarded to the UNICEF/UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases (Grant Nos. 3636/9900727 and AAGG-00-99-00005-31, respectively). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. The authors of this paper alone are responsible for the views expressed in this publication which do not necessarily represent the decisions or policies of the World Health Organization.
Competing Interests: AR is employed by WHO/TDR, the organization which administered the USAID and BMGF grants that funded the study. AR is Secretary of the New Diagnostics Working Group, Stop TB Partnership.
Citation: Cuevas LE, Yassin MA, Al-Sonboli N, Lawson L, Arbide I, et al. (2011) A Multi-Country Non-Inferiority Cluster Randomized Trial of Frontloaded Smear Microscopy for the Diagnosis of Pulmonary Tuberculosis. PLoS Med 8(7): e1000443. doi:10.1371/journal.pmed.1000443
Sub-study: "LED Fluorescence Microscopy for the Diagnosis of Pulmonary Tuberculosis: A Multi-Country Cross-Sectional Evaluation"
Funding: This research was funded by the Bill & Melinda Gates Foundation and the United States Agency for International Development through grants awarded to the UNICEF/UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases (Grant Nos. 3636/9900727 and AAGG-00-99-00005-31, respectively). These funders did not play any role in study design, data collection, decision to publish, the analysis or interpretation of the data for the study or preparation of the manuscript. The LUMIN and the QBC Paralens Fluorescence Microscopy Systems were generously provided free of charge by LW Scientific, which also paid the costs of shipping the systems to study sites. No other financial support was provided by LW Scientific or by QBC Diagnostics. Information supplied on the fluorescence microscopy systems by LW Scientific or QBC Diagnostics consisted only of technical support materials that are made available to all purchasers of the units, or that could be obtained via the LW Scientifics or QBC Diagnostics technical help desk. Neither LW Scientific nor QBC Diagnostics were involved in the design, conduct or analysis of the study. Neither were they involved in the preparation of the manuscript or decision to publish the study. The authors of this paper alone are responsible for the views expressed in this publication which do not necessarily represent the decisions or policies of the World Health Organization.
Competing Interests: Andrew Ramsay is employed by WHO/TDR, the institution administering the grants from USAID and the Bill & Melinda Gates Foundation that funded this work. The other authors have declared that no competing interests exist.
Citation: Cuevas LE, Al-Sonboli N, Lawson L, Yassin MA, Arbide I, et al. (2011) LED Fluorescence Microscopy for the Diagnosis of Pulmonary Tuberculosis: A Multi-Country Cross-Sectional Evaluation. PLoS Med 8(7): e1001057. doi:10.1371/journal.pmed.1001057
CONTACT FOR BOTH PAPERS:
Luis E. Cuevas
Special Programme for Research and Training in Tropical Diseases (TDR)
World Health Organization
Avenue Appia 20
1211 Geneva 27
+41 22 791 3852
Luis E. Cuevas
Liverpool School of Tropical Medicine
Child and Reproductive Health
+44 151 7053219
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