The largest-ever analysis of genetic data related to type 1 diabetes has uncovered new genes associated with the common metabolic disease, which affects 200 million people worldwide. The findings add to knowledge of gene networks involved in the origin of this complex disorder, in which patients depend on frequent insulin injections to control their blood sugar levels.
"Genome-wide association studies, as we used here, have been extremely powerful in identifying gene locations involved in the pathogenesis of complex, common diseases," said study leader Hakon Hakonarson, M.D., Ph.D., director of the Center for Applied Genomics at The Children's Hospital of Philadelphia. "The larger the cohort used, the more discoveries we can make, and the more we find intriguing biological pathways offering insight into causes of disease."
The study appears online today in Public Library of Science Genetics (PLoS Genetics).
The genome-wide association study (GWAS), in which Hakonarson collaborated with Constantine Polychronakos, M.D., director of Pediatric Endocrinology at McGill University, was a meta-analysis, investigating combined DNA data from six large publicly available datasets of type 1 diabetes. The six studies included data from approximately 10,000 individuals with the disease and 17,000 control subjects. The databases contained single nucleotide polymorphisms (SNPs)—single-base changes in DNA sequence that serve as signposts for gene mutations associated with them.
SNPs are not disease-causing mutations, but they reside in gene regions associated with the disease, and set the stage for more detailed sequencing studies to pinpoint causative mutations. Previous studies by Hakonarson and colleagues over the past four years had already discovered SNPs related to type 1 diabetes.
In addition to validating results from previous studies, the current research identified, then replicated, three novel SNPs located in regions of considerable biological interest, being involved in protein-protein interactions, inflammation and cell signaling activity. "Our study found SNPs that we had not expected to have any connection to type 1 diabetes," said Hakonarson. "The strongest association among the three SNPs was in the region of the LMO7 gene on chromosome 13. We previously associated another member of the LMO gene family with the childhood cancer neuroblastoma. This gene family plays an important role in protein-protein interactions, but it would not have occurred to anyone that it may be active in type 1 diabetes. GWAS continues to turn up surprising biological associations."
Hakonarson added that follow-up studies will focus on resequencing the regions linked to the SNPs to narrow down causative mutations. Further research will concentrate on investigating how specific mutations function in the development of type 1 diabetes.
The current study does not yet suggest new diabetes treatments, which are expected to evolve from greater understanding of the fundamental biology of the disease. The scientists estimate that researchers have discovered gene regions accounting for roughly two-thirds of the genetic risk of type 1 diabetes. "We're much further along in finding the full genetic architecture in type 1 diabetes than in any other complex disorder," said co-author, Struan F. A. Grant, Ph.D., associate director of the Center for Applied Genomics at Children's Hospital. "But there's clearly much work ahead in translating knowledge to clinical use."
Funding for the study came from the National Institute of Diabetes and Digestive and Kidney Diseases, the Cotswold Foundation, Genome Canada, the Ontario Genomics Institute and the Juvenile Diabetes Research Foundation. The Children's Hospital of Philadelphia also provided an Institutional Development Award to the Center for Applied Genomics.
"A genome-wide meta-analysis of six type 1 diabetes cohorts identifies multiple associated loci," PLoS Genetics, published online Sept. 29, 2011, freely available at http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002293
About The Children's Hospital of Philadelphia: The Children's Hospital of Philadelphia was founded in 1855 as the nation's first pediatric hospital. Through its long-standing commitment to providing exceptional patient care, training new generations of pediatric healthcare professionals and pioneering major research initiatives, Children's Hospital has fostered many discoveries that have benefited children worldwide. Its pediatric research program is among the largest in the country, ranking third in National Institutes of Health funding. In addition, its unique family-centered care and public service programs have brought the 516-bed hospital recognition as a leading advocate for children and adolescents. For more information, visit http://www.chop.edu.
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