image: Nestin immunoreactivity (red) in the neural rosette structures of the Noggin-induced group under confocal microscope is shown. This is a nuclear staining with 4',6-diamidino-2-phenylindole (blue) and the fluorescence stain was Cy3. view more
Credit: <i>Neural Regeneration Research</i>
The difference between Noggin and basic fibroblast growth factor for the neural precursor differentiation from human embryonic stem cells has not been studied. Prof. Xuejin Chen and colleagues from Shanghai Jiao Tong University were the first to utilize 100 µg/L Noggin or 20 µg/L basic fibroblast growth factor in serum-free neural induction medium to differentiate human embryonic stem cells H14 into neural precursors using monolayer differentiation. The researchers found that Noggin has a greater effect than basic fibroblast growth factor on the induction of human embryonic stem cell differentiation into neural precursors by monolayer differentiation, as Noggin accelerates and increases the differentiation of neural precursors. These findings were published in the Neural Regeneration Research (Vol. 8, No. 23, 2013).
Article: " Noggin versus basic fibroblast growth factor on the differentiation of human embryonic stem cells," by Yan Zhang1, Junmei Zhou2, Zhenfu Fang1, Manxi Jiang1, Xuejin Chen1 (1 Department of Laboratory Animal Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China; 2 Tissue Engineering Laboratory, Shanghai Institute for Pediatric Research, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200092, China)
Zhang Y, Zhou JM, Fang ZF, Jiang MX, Chen XJ. Noggin versus basic fibroblast growth factor on the differentiation of human embryonic stem cells. Neural Regen Res. 2013;8(23):2171-2177.
Contact: Meng Zhao
eic@nrren.org
86-138-049-98773
Neural Regeneration Research
http://www.nrronline.org/
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Journal
Neural Regeneration Research