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PUBLIC RELEASE DATE:
13-Feb-2014

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Contact: Lisa Larson
lisa.larson@umassmed.edu
508-856-2000
University of Massachusetts Medical School

Vitamin B12 accelerates worm development

UMass Medical School researchers develop new model for isolating the effects of nutrients on gene expression and physiology

WORCESTER, MA Everyday our cells take in nutrients from food and convert them into the building blocks that make life possible. However, it has been challenging to pinpoint exactly how a single nutrient or vitamin changes gene expression and physiology. Scientists at the University of Massachusetts Medical School have developed a novel interspecies model system that allows these questions to be answered. In a study appearing in the journal Cell, UMMS researchers use this new approach to show how bacterially supplied vitamin B12 changes gene expression, development and fertility in the model organism C. elegans.

"In mammals, micronutrients are provided by a combination of diet and gut flora," said A.J. Marian Walhout, PhD, co-director of the Program in Systems Biology and professor of molecular medicine at UMMS and senior author of the study. "We've developed a powerful approach that can be used to unravel the complex interaction between nutrients, gene expression and physiology by systematically studying both the predator (worm) and the prey (bacteria). With it we can begin to answer important questions about how what we eat affects how we function."

The key to the study was a set of complimentary genetic screens performed on the transparent roundworm C. elegans and two kinds of bacteria that comprised the worm's diet Comamonas and E. coli. In a pair of papers published last year, Walhout and colleagues described dramatic changes in gene expression between worms fed only Comamonas and those fed only E. coli bacteria. Linked to these genetic changes were profound physiological differences between the worms. Comamonas-fed worms developed faster and were less fertile than their E. coli-fed counterparts.

By genetically dissecting the two bacteria and using a special C. elegans strain developed to sense changes to diet-related gene expression, Walhout and colleagues were able to zero in on a set of genes present in Comamonas but absent from E. coli. Further testing confirmed that these genes were responsible for producing vitamin B12 in Comamonas and it was the presence of the micronutrient that accounted for the genetic and physiological differences seen between the worms on different diets.

Importantly, Walhout found that vitamin B12 fulfills two important functions in C. elegans: It helps regulate development through the methionine/SAM cycle, which is needed for the production of cell membranes in new cells. It also alleviates potentially toxic buildups of the short-chain fatty acid propionic acid, which can alter gene expression or harm cells.

"C. elegans fed E. coli are actually vitamin B12 deficient and this reflects only one natural state of the animal," said Walhout. "Because E. coli has been the standard laboratory diet for decades it would be interesting to study other characteristics of the worm, such as behavior, mating and movement, on a vitamin B12 rich diet."

Walhout and colleagues say that this system can also be adapted to identify genetic and physiological changes caused by other micronutrients in C. elegans. With the proper human analogs, it's possible that we could one day predict the precise interaction between diet, gene expression and physiology that occurs when we eat a carrot, hamburger, steak or any other food. Doing so might someday lead to new insights into a variety of conditions or diseases such as high cholesterol, heart disease, diabetes and obesity. It can also be used to explore the precise benefits of bacteria found in gut flora.

"It turns out a single transgenic worm is a powerful tool for exploring the complex interaction between macro and micronutrients, gene expression and physiology," said Emma Watson, a doctoral student in the Walhout Lab and first author on the Cell study.

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About the University of Massachusetts Medical School

The University of Massachusetts Medical School (UMMS), one of five campuses of the University system, is comprised of the School of Medicine, the Graduate School of Biomedical Sciences, the Graduate School of Nursing, a thriving research enterprise and an innovative public service initiative, Commonwealth Medicine. Its mission is to advance the health of the people of the Commonwealth through pioneering education, research, public service and health care delivery with its clinical partner, UMass Memorial Health Care. In doing so, it has built a reputation as a world-class research institution and as a leader in primary care education. The Medical School attracts more than $240 million annually in research funding, placing it among the top 50 medical schools in the nation. In 2006, UMMS's Craig C. Mello, PhD, Howard Hughes Medical Institute Investigator and the Blais University Chair in Molecular Medicine, was awarded the Nobel Prize in Physiology or Medicine, along with colleague Andrew Z. Fire, PhD, of Stanford University, for their discoveries related to RNA interference (RNAi). The 2013 opening of the Albert Sherman Center ushered in a new era of biomedical research and education on campus. Designed to maximize collaboration across fields, the Sherman Center is home to scientists pursuing novel research in emerging scientific fields with the goal of translating new discoveries into innovative therapies for human diseases.



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