Researchers at the University of Texas Medical Branch at Galveston by growing vaginal skin cells outside the body and studying the way they interact with "good and bad" bacteria, think they may be able to better identify the good bacteria that protect women from HIV infection and other sexually transmitted infections.
The health of the human vagina depends on a symbiotic/mutually beneficial relationship with "good" bacteria that live on its surface feeding on products produced by vaginal skin cells. These good bacteria, in turn, create a physical and chemical barrier to bad bacteria and viruses including HIV.
A publication released today from a team of scientists representing multiple disciplines at UTMB and the Oak Crest Institute of Science in Pasadena, Calif., reports a new method for studying the relationship between the skin cells and the "good" bacteria.
The researchers are the first to grow human vaginal skin cells in a dish in a manner that creates surfaces that support colonization by the complex good and bad communities of bacteria collected from women during routine gynecological exams. The bacteria communities have never before been successfully grown outside a human.
The research group led by Richard Pyles at UTMB reports in the journal PLOS One that by using this model of the human vagina, they discovered that certain bacterial communities alter the way HIV infects and replicates. Their laboratory model will allow careful and controlled evaluation of the complex community of bacteria to ultimately identify those species that weaken the defenses against HIV. Pyles also indicated that this model "will provide the opportunity to study the way that these mixed species bacterial communities change the activity of vaginal applicants including over-the-counter products like douches and prescription medications and contraceptives. These types of studies are very difficult or even impossible to complete in women who are participating in clinical trials."
In fact, the team's report documented the potential for their system to better evaluate current and future antimicrobial drugs in terms of how they interact with "good and bad" bacteria. In their current studies a bacterial community associated with a symptomatic condition called bacterial vaginosis substantially reduced the antiviral activity of one of the leading anti-HIV medicines.
Conversely, vaginal surfaces occupied by healthy bacteria and treated with the antiviral produced significantly less HIV than those vaginal surfaces without bacteria treated with the same antiviral. Dr. Marc Baum, the lead scientist at Oak Crest and co-author of the work, stated "this model is unique as it faithfully recreates the vaginal environment ex vivo, both in terms of the host cellular physiology and the associated complex vaginal microbiomes that could not previously be cultured. I believe it will be of immense value in the study of sexually transmitted infections."
Original findings published in PLOS One Journal, March 27, 2014: "Cultivated vaginal microbiomes alter HIV-1 infection and antiretroviral efficacy in colonized epithelial multilayer cultures."
Authors: Richard B. Pyles, Kathleen L. Vincent, Marc M. Baum, Barry Elsom, Aaron L. Miller, Carrie Maxwell, Tonyia D. Eaves-
Pyles, Guangyu Li, Vsevolod L. Popov, Rebecca J. Nusbaum and Monique R. Ferguson.
The University of Texas Medical Branch
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ABOUT UTMB HEALTH: Texas' first academic health center opened its doors in 1891 and today comprises four health sciences schools, three institutes for advanced study, a research enterprise that includes one of only two national laboratories dedicated to the safe study of infectious threats to human health, and a health system offering a full range of primary and specialized medical services throughout Galveston County and the Texas Gulf Coast region. UTMB Health is a component of the University of Texas System and a member of the Texas Medical Center.
ABOUT THE OAK CREST INSTITUTE OF SCIENCE: Founded in 1998, the Oak Crest Institute of Science is an innovative chemistry research and education center that advances the understanding of environmental and medical science through rigorous academic laboratory programs. Oak Crest originated with the goal of providing an academic science research environment accessible to participants spanning a broad diversity of educational levels from high school to post graduate. Oak Crest is dedicated to solving some of the toughest biomedical and environmental problems of today while cultivating tomorrow's scientists one individual at a time.
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