The development is a step towards commercial production of a source of fuel that could one day provide an alternative to fossil fuels.
Propane is an appealing source of cleaner fuel because it has an existing global market. It is already produced as a by-product during natural gas processing and petroleum refining, but both are finite resources. In its current form it makes up the bulk of LPG (liquid petroleum gas), which is used in many applications, from central heating to camping stoves and conventional motor vehicles.
In a new study, the team of scientists from Imperial College London and the University of Turku in Finland used Escherichia coli to interrupt the biological process that turns fatty acids into cell membranes. The researchers used enzymes to channel the fatty acids along a different biological pathway, so that the bacteria made engine-ready renewable propane instead of cell membranes.
Their ultimate goal is to insert this engineered system into photosynthetic bacteria, so as to one day directly convert solar energy into chemical fuel.
The results of the study are published in the journal Nature Communications.
Dr Patrik Jones, from the Department of Life Sciences at Imperial College London said: "Although this research is at a very early stage, our proof of concept study provides a method for renewable production of a fuel that previously was only accessible from fossil reserves. Although we have only produced tiny amounts so far, the fuel we have produced is ready to be used in an engine straight away. This opens up possibilities for future sustainable production of renewable fuels that at first could complement, and thereafter replace fossil fuels like diesel, petrol, natural gas and jet fuel."
The scientists chose to target propane because it can easily escape the cell as a gas, yet requires little energy to transform from its natural gaseous state into a liquid that is easy to transport, store and use.
"Fossil fuels are a finite resource and as our population continues to grow we are going to have to come up with new ways to meet increasing energy demands. It is a substantial challenge, however, to develop a renewable process that is low-cost and economically sustainable. At the moment algae can be used to make biodiesel, but it is not commercially viable as harvesting and processing requires a lot of energy and money. So we chose propane because it can be separated from the natural process with minimal energy and it will be compatible with the existing infrastructure for easy use" added Dr Jones.
Using E. coli as a host organism, the scientists interrupted the biological process that turns fatty acids into cell membranes. By stopping this process at an early stage they could remove butyric acid, a nasty smelling compound that is an essential precursor for propane production.
To interrupt the process, the researchers discovered a new variant of an enzyme called thioesterase which specifically targets fatty acids and releases them from the natural process. They then used a second bacterial enzyme, called CAR, to convert butyric acid into butyraldehyde. Finally, they added a recently discovered enzyme called aldehyde-deformylating oxygenase (ADO), which is known to naturally create hydrocarbons, in order to form propane.
Previous attempts to use the ADO enzyme have proved disappointing as scientists have been unable to harness the natural power of the enzyme to create cleaner fuel. But the scientists at Imperial discovered that by stimulating ADO with electrons they were able to substantially enhance the catalytic capability of the enzyme, and ultimately produce propane.
The level of propane that the scientists produced is currently one thousand times less than what would be needed to turn it into a commercial product, so they are now working on refining their newly designed synthetic process. Dr Jones said: "At the moment, we don't have a full grasp of exactly how the fuel molecules are made, so we are now trying to find out exactly how this process unfolds. I hope that over the next 5-10 years we will be able to achieve commercially viable processes that will sustainably fuel our energy demands."
This research was funded by a grant from the European Research Council.
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Notes to editors:
1. Jones, P, R. et al. 'An engineered pathway for the biosynthesis of renewable propane'. Nature Communications, September 2014.
Once embargo has lifted on Tuesday 2 September 2014 at 1600 BST / 1100 US Eastern time, the paper can be downloaded at: http://dx.
2. About Imperial College London
Consistently rated amongst the world's best universities, Imperial College London is a science-based institution with a reputation for excellence in teaching and research that attracts 14,000 students and 6,000 staff of the highest international quality. Innovative research at the College explores the interface between science, medicine, engineering and business, delivering practical solutions that improve quality of life and the environment - underpinned by a dynamic enterprise culture.
Since its foundation in 1907, Imperial's contributions to society have included the discovery of penicillin, the development of holography and the foundations of fibre optics. This commitment to the application of research for the benefit of all continues today, with current focuses including interdisciplinary collaborations to improve global health, tackle climate change, develop sustainable sources of energy and address security challenges.
In 2007, Imperial College London and Imperial College Healthcare NHS Trust formed the UK's first Academic Health Science Centre. This unique partnership aims to improve the quality of life of patients and populations by taking new discoveries and translating them into new therapies as quickly as possible.