Public Release: 

Getting closer to using beer hops to fight disease

American Chemical Society

SAN DIEGO, March 13, 2016 -- Hops, those little cone-shaped buds that give beer its bitter flavor, pack a surprisingly healthful punch. They are widely studied for their ability to halt bacterial growth and disease. Now, researchers report that they are close to synthesizing the healthful hops compounds in the lab. This advance could one day help scientists create medicines from these compounds without having to extract them from plants.

The researchers present their work today at the 251st National Meeting & Exposition of the American Chemical Society (ACS). ACS, the world's largest scientific society, is holding the meeting here through Thursday. It features more than 12,500 presentations on a wide range of science topics.

"When researchers extract healthful chemicals from hops, they first have to determine whether they have separated out the specific compounds they're interested in," says Kristopher Waynant, Ph.D., who leads the project. "But if you can figure out how to make these compounds from scratch, you know they are the right ones."

The compounds Waynant is interested in are humulones, which are alpha acids that have anticancer and anti-inflammatory properties, and lupulones, which are beta acids that have equally important but not as well-understood biological effects. Scientists ultimately want to harness these activities to potentially improve human health. But before humulones and lupulones can be turned into effective pharmaceuticals, scientists must confirm that they are extracting the proper acids from hops. In theory, they can do this by separating the acids with high performance liquid chromatography, but results from this method need to be compared to analytical standards, which do not yet exist for the individual compounds.

In order to create a library of such analytical standards, Waynant and undergraduate student Lucas Sass, both at the University of Idaho, are attempting to synthesize a set of humulones and lupulones in the lab.

The researchers start with phloroglucinol -- a commercially available compound that is derived from plants. So far, they have been developing multi-step processes to synthesize three types of humulones. They begin with acylation and prenylation steps, and then use transition-metal catalysis with a chiral ligand to create asymmetric products. The team is still working on tweaking the final step of converting the intermediate products in the desired humulones efficiently.

"Unfortunately, the first few pathways I proposed were not the best or most efficient," Waynant admits. "But Lucas has gone through the literature and analyzed different ways to perform each of these steps to get the best results."

Currently, Sass is making progress on optimizing the first two steps. "It's been a lot of trial and error," Sass says. "But it's so exciting when an approach finally works."

The researchers say they are getting very close to optimizing the complete method for producing humulones in the lab.

In the future, Waynant's team will continue the project to synthesize a broader range of humulones and lupulones. He also plans to reach out to and collaborate with biologists and medical researchers to develop active agents for pharmaceuticals to treat cancers or inflammatory diseases from these beer-inspired compounds.

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A press conference on this topic will be held Monday, March 14, at 9 a.m. Pacific time in the San Diego Convention Center. Reporters may check-in at Room 16B (Mezzanine) in person, or watch live on YouTube http://bit.ly/ACSliveSanDiego. To ask questions online, sign in with a Google account.

The American Chemical Society is a nonprofit organization chartered by the U.S. Congress. With more than 158,000 members, ACS is the world's largest scientific society and a global leader in providing access to chemistry-related research through its multiple databases, peer-reviewed journals and scientific conferences. Its main offices are in Washington, D.C., and Columbus, Ohio.

The researchers acknowledge funding from the University of Idaho and the National Institutes of Health IDeA Networks of Biomedical Research Excellence program.

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Title

Exploring Hops Chemistry: Towards Efficient Asymmetric Syntheses of Humulones and Lupulones

Abstract

Humulones and lupulones have affirmed themselves as key ingredients in the multi-billion dollar brewing industry. Originally exploited for their bacteriostatic properties, these compounds also exhibit high levels of biological activity against a variety of diseases. Our investigations are towards developing a synthetic route to a library of humulones, lupulones, and their derivatives as possible biologically active agents against myriad diseases. Prenylation of acylphloroglucinol yields a mix of lupulones and bisprenylated acylphloroglucinols. The key step in our efficient syntheses of individual humulones is a copper-mediated asymmetric oxidative dearomatization involving chiral sparteine ligands and an oxo-copper species developed by Porco, et al. Our investigations are also leading towards the diasteromeric adhumulones and determining the chirality of natural adhumulone. Active agents may lead to a transformation in hops as sources to the next generation of medicines.

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