Figure 1. (IMAGE)

Nanjing Agricultural University The Academy of Science

Figure 1.

Caption

GUS/LUC dual reporter system for detecting CRISPRa efficacy. (a) CRISPRa construct design for OsTRP-like and OsCCR1 genes. (b)Reporter construct design for OsTRP-like and OsCCR1 promoters. (c) CRISPRa efficiency measurement using two reporter genes, GUS and LUC,in Arabidopsis protoplasts. Bar charts represent mean ± sd (n = 3 three independent protoplast pools), and asterisks represent significant difference between groups determined by Student’s t-test: ∗∗∗, P ≤ 0.001.

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Horticulture Research

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