Working principle of E2E-BPF microscopy. (IMAGE)
Caption
a, An axi-symmetric binary phase filter (BPF) and image reconstruction network are jointly learned through the physics-informed neural network. The learned BPF is fabricated and inserted in a pupil plane in an optical microscope, which produces projected volumetric image over the extended depth range. The acquired image is subsequently fed into the jointly learned reconstruction network to generate high-resolution, high-contrast image over the extended DoF. b, The viability of E2E-BPF microscope was further demonstrated by imaging a large-scale mouse kidney tissue section stained with fluorescent dyes with no refocusing (DAPI-labelled nuclei, blue; AF488-labelled tubules, green; AF568-labelled F-actin, red). OBJ: microscope objective; NA: numerical aperture; TL: tube lens; CAM: camera.
Credit
by Baekcheon Seong, Woovin Kim, Younghun Kim, Kyung-A Hyun, Hyo-Il Jung, Jong-Seok Lee, Jeonghoon Yoo, and Chulmin Joo
Usage Restrictions
Credit must be given to the creator.
License
CC BY