Physiological changes of banana fruit and molecular characterization of MabHLH28. (IMAGE)
Caption
Physiological changes of banana fruit and molecular characterization of MabHLH28. (A) Phenotype of bananas under natural ripening and ethylene-induced ripening. (B) Variation of ethylene production, fruit firmness, hue angle, and starch content during ripening. Data represent the mean ± SE of six biological replicates (**P < 0.01). (C) Expression of MabHLH28 in banana fruit with and without (control) ethylene treatment. Relative expression level of MabHLH28 was analysed RT-qPCR. Each value is mean ± SE of three replicates (**P < 0.01). (D) MabHLH28 promoter activity in response to ethylene. Each value is mean ± SE of six replicates (**P < 0.01). (E) Subcellular localization of MabHLH28 in tobacco leaf cells. NLS-mCherry serves as a nuclear localization signal marker; bar, 25 μm. (F) Transcriptional activity of MabHLH28 in yeast cells. The pGBKT7 empty vector (BD) was used as a negative control and pGBKT7 -53 + pGADT7-T as a positive control. (G) Transactivation of MabHLH28 in tobacco leaf cells. The ratio of LUC/REN of the empty 62SK-BD vector (negative control) was set to 1. 62SK-BD-VP16 was used as a positive control. Each value is mean ± SE of six replicates. Different letters indicate significant differences at the P < 0.05 level.
Credit
Horticulture Research
Usage Restrictions
Credit must be given to the creator.
License
CC BY