BoNT/Aintoxication of LAN5 cells induces massive tRF changes. (IMAGE)
Caption
BoNT/Aintoxication of LAN5 cells induces massive tRF changes. (A) Experimental design: 2×106 LAN5 cells/well were intoxicated by 10,000 MsLD50/mL BoNT/A, and small RNA-seq profiles from these cells were compared to nontreated (NT) cells, revealing differences in miRNAs and tRFs. Volcano plots of DE transcriptomes (FDR < 0.05) were generated from three biological triplicates per treatment. Differential expression analysis was performed using the EdgeR tool. (B) DE miRNAs. (C) Schematic representation of tRNA cleavage. (D) DE tRFs. (E) Levels of tRF-5 LysTTT were drastically elevated in BoNT/A-intoxicated LAN5 cells. Data represent N = 3 biological replicates; ∗P < 0.05. (F, G, H) Classification of DE tRFs by fragment type, corresponding amino acid, and length.
Credit
Hermona Soreq
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