Figure 1 (IMAGE)
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Functional analysis of the LEM-3 protein and the roles of its domains using the C. elegans model. (A) Evidence shows that LEM-3 accumulates at DNA bridges remaining between daughter cells during the final stages of cell division and contributes to their resolution. This was observed through fluorescence microscopy. (B) LEM-3 function was analyzed across various developmental stages using the C. elegans model, elucidating the mechanism by which DNA bridges are severed during cell division. (C) LEM-3 localizes to the midbody to recognize and cleave DNA bridges. Structural prediction and cleavage assays revealed that the LEM-like domain is responsible for DNA recognition, while the GIY-YIG domain directly mediates DNA cleavage.
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Institute for Basic Science
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