Preosteoblast-specific deletion of NELL2 reduced bone mass in female mice. (IMAGE)
Caption
Preosteoblast-specific deletion of NELL2 reduced bone mass in female mice. a–f Cancellous bone mass at proximal tibial metaphysis in 3-month-old female mice was analyzed using μCT. a Representative reconstruction images are shown. b–f Histomorphometric parameters of cancellous bone were quantified, n = 10. g, h Serum levels of PINP and CTX-1 were measured using ELISA, n = 10. i ABH/OG staining was conducted. Scale bar: 200 μm (upper panel); 50 μm (lower panel). j, k The numbers and area percentage of adipocytes were quantified, n = 6. l Representative images of osteocalcin IHC staining are shown. m, n The numbers and surface percentage of trabecular Ocn+ osteoblasts were quantified, n = 6. o Representative images of calcein double labeling are shown. p, q MAR and BFR/BS were quantified, n = 7. r Representative images of TRAP staining are shown. s, t The numbers and surface percentage of osteoclasts were quantified, n = 6. Scale in (l, o, r): 50 μm. Data are mean ± SD. Comparisons were conducted using Student’s t test, *P < 0.05, **P < 0.01; ns: no significance.
Credit
Bone Research
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