Schematic depiction of the SG 3D culture workflow as spheroid forming platforms using the hanging drop technique. (IMAGE)

West China Hospital of Sichuan University

Schematic depiction of the SG 3D culture workflow as spheroid forming platforms using the hanging drop technique.

Caption

AG, AGC, and AGHA hydrogels were synthesized and mechanically characterized. Next, human SG cells (cultured in the 2D platform) were harvested with trypsin, counted, and mixed separately within hydrogels. Then, drops of 30 µL (3D models) were created by the hanging drop method. The 3D models were thermogelled, ionically crosslinked, and cultured for 14 days. The formation of spheroids was tracked through time by confocal microscopy imaging. Based on hydrogel performance in promoting the formation of highly viable, proliferative, and large acinar spheroids, the AGHA gel was selected to evaluate the expression and localization of key SG proteins and functionality in the produced spheroids. AGHA gels were used to expand and foster functional SFU.

Credit

International Journal of Oral Science

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CC BY

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