Targeting PCNA/AR interaction inhibits AR-mediated signaling in castration resistant prostate cancer cells (IMAGE)
Caption
Figure 8: Dihydrotestosterone regulates cyclin A2 expression in AR-FL expressing cells. (A) Cell extracts from androgen dependent LNCaP (AR-FL+) cells and 4 lines of CRPC cells LNCaP-AI (AR-FL+), 22Rv1(AR-FL+/AR-V7+), R1-D567 (ARv567es), and PC-3(AR−) were prepared. LNCaP or R1-D567 cells were cultured in stripped medium for 1 day and then treated without or with dihydrotestosterone (DHT) (B), as well as R9-AR-PIP (20 μM) or PCNA-I1S (1 μM) (C) for 24 hours. The cell extracts from these cells were subjected to immunoblot analysis using AR, cyclin A, and GAPDH antibodies.
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Copyright: © 2025 Lu and Dong. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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