Impacts of the factors that affect DSB repair pathway choice on the editing efficiency in tomato. (IMAGE)
Caption
Impacts of the factors that affect DSB repair pathway choice on the editing efficiency in tomato. A, Plasmid map of the GT tool used in the study. The GT construct was cloned into a geminiviral replicon vector with the boundaries of two long intergenic sequences (LIRs) and one short intergenic sequence (SIR). The replicon is autonomously replicated with the support of the Rep protein/expression cassette. B–C, The impacts of KUDN and BRCA1 on GT (B) and indel mutation (C) efficiency at the callus stage. The efficiencies were assessed by targeted NGS using 21-dpt cotyledon/callus samples. D, The protein expression levels of ttLbCas12a and its KUDN and BRCA1 fusions. Western blot membranes showing bands of the Cas proteins with expected sizes and expression cassettes. Actin levels were used as loading controls. P-values of the t-test for pairwise comparison between the relative protein levels of ttLbCas12a and the fusions are indicated on the top of the bars. All data points are shown as dots in the plots in (B–D). The expression cassettes and Cas configurations are at the bottom of the bars.
Credit
Horticulture Research
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