Universal Amplification-Free RNA Detection by Integrating CRISPR-Cas10 with Aptameric Graphene Field-Effect Transistor (IMAGE)
Caption
- A collaborative biosensing system is developed for amplification-free RNA/miRNA detection by integrating Type III CRISPR-Cas10 system with a graphene field-effect transistor (GFET).
- Continuous cleavage of ssDNA by the mutant CRISPR-Cas10 effector complexes and high charge density of hairpin DNA reporters on the GFET channel enable the detection limit to reach the level of aM.
- A universal sensing detection platform is established to directly detect the medium-length RNAs and miRNAs in clinical samples with the recognition capability of single nucleic acid.
Credit
Mingyuan Sun, Zhenxiao Yu, Shuai Wang, Jiaoyan Qiu, Yuzhen Huang, Xiaoshuang Chen, Yunhong Zhang, Chao Wang, Xue Zhang, Yanbo Liang, Hong Liu, Qunxin She, Yu Zhang, Lin Han.
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License
CC BY-NC-ND