Evolution of Kp067 to generate phage resistance (IMAGE)
Caption
(A) Evolutionary trajectory of Kp067 examined under sequential exposure to each of the three phages. Visible plaque represents phage sensitivity, while no plaque represents phage resistance. The orange-colored phages represent the Podoviruses phage ΦRCIP0041, while the green-colored ones represent the Myoviridae phage ΦRCIP0002, and the purple-colored ones represent the Siphoviridae phage ΦRCIP0070. R, resistance to phage; S, sensitivity to phage. (B) Schematic representation of IS5 insertion sites in Kp067 phage-resistant mutants. The arrows in colors of blue, tawny, and green, respectively, represent the wcaJ, gmhB, and wbbY, the corresponding genes inserted by IS5, while the red arrows represent IS5. The numbers under the genes represent the coding regions on the chromosome of Kp067 for these three genes: wcaJ, 1773485..1774888; gmhB, 4327107..4327673; and wbbY, 4713063..4715285. The numbers above these genes donate the insertion site of the IS5 element for each gene, for example, 1282; the IS5 inserted into the 1282th bp of the wcaJ gene.
Credit
Jie Feng, Institute of Microbiology, Chinese Academy of Sciences
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