LAMP-LFB Assay for Rapid Differentiation of Monkeypox Virus Strains (IMAGE)
Caption
Schematic mechanism of the MPXV-LAMP assay. (A) The MPXV-LAMP-LFB assay uses loop-mediated isothermal amplification (LAMP) with specific primers to detect Monkeypox virus strains. FIP and LF primers, labeled with hapten and biotin, produce detectable amplicons monitored by a lateral flow biosensor (LFB). (B) This assay distinguishes between Central and West African strains, providing rapid, sensitive, and specific results. (C) It involves two LAMP reactions, generating digoxin/biotin and FAM/biotin labeled amplicons. The LFB visually interprets results, indicating positive or negative outcomes based on the presence of test lines for each strain, thus offering a practical tool for point-of-care diagnostics.
Credit
Abhimanyu Thakur.
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