Longitudinal plastic sections (7 mM thick) of R108 and enod40-1-2/1 nodules 21 days after inoculation with S. mellilot (IMAGE)
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Figure 2. enod40-1-2/1 nodules have wild type-like morphology and MtENOD40 and MtNF-YA1 have similar spatial expression patterns during nodule primordium formation. A. Longitudinal plastic sections (7 mM thick) of R108 and enod40-1-2/1 nodules 21 days after inoculation with S. melliloti 2011, counter-stained with toluidine blue. M – meristem, IZ- infection zone, FZ – fixation zone. Arrows point to nodule vascular bundles Scale bars: 100 µM. B, C. Representative images of RNA in situ localization of MtENOD40 (A) and MtNF-YA1 (B) transcripts in nodule primordia at developmental stage V. Both genes are expressed in pericycle and cortical cell layers 4 and 5. c3, cortical cell layer 3; c4/5 cortical cell layers 4 and 5; en, endodermis; pc, pericycle. Scale bars: 100 µM.
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Published in Wijsmana et al 2025 in PNAS
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