Figure 3. Schematic diagram of [13N]N2 flow path within the plant root delivery system (IMP2RIS) during the [13N]N2 delivery (violate arrowed lines) and evacuation (green arrowed lines) operational modes. (IMAGE)

Nanjing Agricultural University The Academy of Science

Figure 3. Schematic diagram of [13N]N2 flow path within the plant root delivery system (IMP2RIS) during the [13N]N2 delivery (violate arrowed lines) and evacuation (green arrowed lines) operational modes.

Caption

(a) During the introduction of [13N]N2 gas, valve #1 is closed and valve #2 and cyclotron valve are opened, which allows for the [13N]N2 gas to be transferred to the pot through the pot gas-entry port, transported into the soil via the riser tubes and the gas then exits the system by being directed into a waste bag. (b) During evacuation, the cyclotron's [13N]N2 supply line is shut off, valve #2 is closed, and valve #1 is opened. Prior to the initiation of the evacuation of residual gas from the soil, the tubing from the quick push connect on valve #2 is manually disengaged. This will allow for room air to be pulled into the pot through the gas port on the pot lid, flushing out the residual radioactive gas within the soil column through the pot risers, and then discharging it into the waste bag.

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