Cost-effective oligo system design schematic. (IMAGE)

Nanjing Agricultural University The Academy of Science

Cost-effective oligo system design schematic.

Caption

Cost-effective oligo system design schematic. Each synthetic oligo consists of two oligos (oligo-A and oligo-B), one forward primer (Primer-A), and two reverse primers (Primer-B and Primer-C). (A) Oligo library filtering process. The density of the oligo library is 2.5 oligos per kb. (B) Schematic selection of oligo A and B. Synthetic oligos with intervals shorter than 200 kb are omitted for FISH performance. (C) Scheme of primer introducing and PCR amplification. Primer-A and Primer-C are used to amplify the whole pool of different colors/modification probe oligos (C1). Primer-A and Primer-B are used to amplify one individual chromosome or segment (C2). Primer-B and Primer-B′ correspond to distinct sections of the primer, respectively.

Credit

Horticulture Research

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