Disruption of ClOSD1 leads to both somatic and gametic ploidy doubling in watermelon. (IMAGE)

Nanjing Agricultural University The Academy of Science

Disruption of ClOSD1 leads to both somatic and gametic ploidy doubling in watermelon.

Caption

Disruption of ClOSD1 leads to both somatic and gametic ploidy doubling in watermelon. (A) Phylogenetic tree depicting OSD1 predicted proteins from 19 monocot and dicot plant species. (B) In situ hybridization analysis of ClOSD1 at different stages of watermelon male flower development. Scale bars: 50 μm. (C) Comparison of WT, Closd1-1 and Closd1-2 mutant plant phenotypes. (D) Schematic representation of ClOSD1 gene structure, target sequence, and editing results in different Closd1 mutants. (E) Flow cytometric analysis of Closd1-1 and Closd1-2 plants ploidy. (F) Chromosome count statistics in wild-type, Closd1-1 and Closd1-2 plants via immunofluorescence assay. (G) Male meiotic product and activity analysis in Closd1-1, Closd1-2, and WT plants. Scale bar: 20 μm. (H) Proportions of different male meiotic products in WT, Closd1-1, and Closd1-2 mutant. (I) Pollen viability analysis using Alexander staining in mature pollen of WT, Closd1-1, and Closd1-2 plants. Scale bar: 100 μm. (J) Statistics of mature pollen diameter in WT, Closd1-1 and Closd1-2. (K) Ploidy determination of WT, Closd1-1, and Closd1-2 mutant leaves and pollen. (L) Fruits and seeds of each hybrid combination of the different Closd1 mutants. (M) Flow cytometry analysis of the progeny from reciprocal crosses between Closd1-2 mutant.

Credit

Horticulture Research

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