Adaptive Optics-Assisted Long-Term 3D Dual-Modal Imaging (IMAGE)

Smart Computational Imaging Laboratory (SCILab), Nanjing University of Science and Technology

Adaptive Optics-Assisted Long-Term 3D Dual-Modal Imaging

Caption

In this article, the researchers introduce AO-FIDT, a novel long-term, high-spatiotemporal-resolution 3D fluorescence and intensity diffraction tomography microscopy technique. This method employs an iterative ptychographic approach to decouple 3D RI and wavefront aberrations from unlabeled intensity images. By feeding real-time wavefront aberrations back into the system's PSF, it synchronously corrects 3D fluorescence results and improves reconstruction quality. As the first AO-assisted 3D dual-modal imaging application, AO-FIDT effectively addresses the traditional challenge of reconciling long-term imaging with high spatiotemporal resolution, representing a significant advancement in 3D dual-modal microscopy.

Credit

Ning Zhou et al. from Nanjing University of Science and Technology, Nanjing, China Image source: https://onlinelibrary.wiley.com/cms/asset/83eca2ed-3c65-4ec9-993e-0a62835d9e05/lpor202570059-gra-0001-m.jpg

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