Decoding the kiss-shrink-run mechanism with time-resolved cryo-ET. (IMAGE)

University of Science and Technology of China

Decoding the kiss-shrink-run mechanism with time-resolved cryo-ET.

Caption

A millisecond-precision time-resolved cryo-ET system, combining plunge-freezing with optogenetic stimulation (top left), captured distinct intermediate states of SV exocytosis in intact hippocampal synapses (top right). With population analysis and subtomogram averaging, a kiss-shrink-run sequence of SV exocytosis was revealed as the prominent SV exocytosis and recycling pathway in hippocampal synapses (bottom). AP, action potential.

Credit

Prof. Bi’s team

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License

CC BY-ND

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