Histological and cellular characterization of zebrafish larval development. (IMAGE)
Caption
Histological and cellular characterization of zebrafish larval development. a Morphological comparison of an adult zebrafish (left) and 5 days post-fertilization (dpf) larva (right). Scale bar: 100 µm. b Left: schematic of the paraffin-embedding and sectioning protocols for zebrafish larvae. Right: histological sections highlighting the retina, optic nerve, and brain (green boxes indicate magnified regions). Scale bars: larval overview, 50 µm; retina, 20 µm; optic nerve, 10 µm; brain, 20 µm. c Left: key apparatus for larval embedding. Right: schematic of larval zebrafish immobilized in low-melting-point agarose for imaging. The diagram illustrates different embedding orientations. Anterior (A), posterior (P), dorsal (D), and ventral (V) anatomical directions are indicated.
Credit
Eye and Vision
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CC BY