Application of Crr5-specific marker in molecular-assisted breeding. (IMAGE)

Nanjing Agricultural University The Academy of Science

Application of Crr5-specific marker in molecular-assisted breeding.

Caption

Application of Crr5-specific marker in molecular-assisted breeding. (A) Development of the resistant line R16-Crr5 using the Crr5-specific marker-assisted selection. DH40 was used as the donor parent and susceptible R16 was used as the recurrent parent. (B) Validation of the presence of Crr5 in the R16-Crr5 genome by PCR. (C) Clubroot phenotype of R16 and R16-Crr5 after inoculation of PbXY-2 isolate. Gall formation was examined and photographed at 35 days after inoculation. (D, E) Validation of the Crr5-specific marker in (DH40 × DH199)-F2 population (D) and in a natural population (E).

Credit

Horticulture Research

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