H3/H4-Wo protein complexes enhance the transcriptional activity of Wo and Wox3b genes. (IMAGE)
Caption
H3/H4-Wo protein complexes enhance the transcriptional activity of Wo and Wox3b genes. (a) Schematic overview of vector constructs used in DLR assays. The promoters of Wo, Wox3b, and MX1 were cloned upstream of the LUC gene in the pGreen II-0800-LUC reporter vector (pGreen II-proWo, pGreen II-proWox3b, and pGreen II-proMX1). Full-length Wo, H3, and H4 genes were fused downstream of the 35S promoter in the pKCo effector vector (35S:Wo, 35S:H3, and 35S:H4). These reporter and effector constructs were coinfiltrated in tobacco leaves via agroinfiltration. An empty pKCo vector (35S:EV) served as a negative control. DLR assays were conducted 2 days postinfiltration. (b) Transactivation of Wo, Wox3b, MX1, and HD8 promoters by H3/H4-Wo complexes. LUC and Renilla luciferase (REN) activities were measured, with 35S:EV serving as a negative control. LUC activity was calculated as the ratio of LUC to REN and normalized to that of the control. Data are presented as the mean (±SE) of eight biological replicates. Different letters indicate statistically significant differences (P < 0.05, one-way ANOVA with Tukey’s post hoc test). (c) Proposed model of H3/H4-Wo protein complexes in tomato trichome development. Left: Wo protein, synthesized via its own transcription, directly regulates the expression of trichome development-related genes, including Wox3b, MX1, and HD8. Center-left: H3 and H4 proteins interact with Wo to enhance Wo expression, thereby promoting Wox3b, MX1, and HD8 expression. Center-right: More H3/H4-Wo complexes bind to the Wox3b promoter, further enhancing Wox3b expression. Right: H3 and H4 proteins may interact with other regulatory proteins, potentially modulating the expression of other trichome development genes.
Image link: https://academic.oup.com/view-large/figure/512057160/uhaf008f6.tif
Credit
Horticulture Research
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