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Workflow of mouse cardiomyocyte isolation and patch clamp recording. A Langendorff perfusion system. Schematic representation of the Langendorff method for isolating cardiomyocytes. The heart is excised from the mouse, followed by perfusion with an enzyme-containing solution to dissociate cells. B Heart digestion. Image of the mouse heart being digested using the Langendorff perfusion system. The perfusion needle is inserted into the aorta, and the heart is perfused with collagenase solution. C Isolated cardiomyocytes. Light microscopy image showing isolated adult mouse cardiomyocytes. The cells are shown to disperse after enzymatic digestion. Scale bar, 50 μm. D Patch clamp setup. Image of a cardiomyocyte being patched with a glass micropipette. The cell is positioned under an inverted microscope, and the pipette is used to form a high-resistance seal for recording. Scale bar, 50 μm
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HIGHER EDUCATON PRESS
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