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The working principle and resolution of three fluorescence microscopy techniques: MINFLUX, RESI and ONE, (Reinhardt et al. 2023; Sahl et al. 2024; Shaib et al. 2024) respectively. A The donut-shaped excitation spot in MINFLUX used to achieve precise localization. B MINFLUX reconstructions of polyproline end-to-end distances. C RESI achieves sub-nanometer resolution by using orthogonal DNA sequences (blue and green) and sequential acquisition as in Exchange-PAINT. D The RESI technique resolves adjacent docking strands and has an average accuracy of 1.2 Å for single base pair backbone distances, with Euclidean distances of 8.5 ± 1.7 Å calculated from individual localizations. E The expansion and labeling process of biological samples in ONE method. F Imaging of three immunoglobulins (IgG, IgA, IgM) by ONE
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HIGHER EDUCATON PRESS
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