The expression of NSUN2 was upregulated in hypertrophic hearts. (IMAGE)
Caption
(a) RNA sequencing of C57BL/6 mice heart tissue that underwent Sham or TAC surgery at ten weeks exclusively analyzed the expression of m5C-related enzymes (n = 3). (b) WB analysis of NSUN2 in the hearts of Sham-operated and TAC-treated hearts at different time points (including one (TAC-1w), three (TAC-3w), six (TAC-6w), and ten weeks (TAC-10w)) (n = 5). (c) Representative immunofluorescence images of NSUN2 in the heart section (n = 9). (d) Quantification of qRT-PCR data of NSUN2 in Sham-operated and TAC-treated ten weeks mice hearts (n = 5). (e) WB analysis was used to detect the level of NSUN2 protein in the hearts of the differential group of mice (n = 5). (f) Quantification of qRT-PCR data of NSUN2 in Ang II-treated CMs compared to that in the control group (n = 5). (g) WB analysis of NSUN2 in non-HF and HF human heart samples (n = 3). GAPDH: glyceraldehyde-3-phosphate dehydrogenase; DAPI: 4′,6-diamidino-2-phenylindole.
Credit
Yingqi Liu, Fan Wu, Kuiwu Liu, et al.
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