Characterization of RMF tissue cytoarchitecture. (IMAGE)
Caption
(a) Timeline of main steps for generating RMF pellets with a diameter of 4 mm from human adipose tissues. Scale bars, 5 mm. (b) SEM and (c) HE images of the lipoaspirate tissue, microfat tissue, RMF, and hemi-sectioned RMF pellets (4 mm). Scale bars, (b) 100 μm, (c) 200 μm. (d) Representative immunofluorescence images showing expression of proliferation marker Ki67 (green) and mature adipocyte marker perilipin-2 (red) in native adipose tissues and RMF tissues. Scale bars, 50 μm. (e) Immunohistochemical images of matrix proteins, including collagen type IV (Col IV), laminin, fibronectin, and Col I, in native adipose tissues and RMF tissues. Scale bars, 200 μm. (f) Quantification of DNA content per 20 mg of RMF tissues during the three weeks of self-reaggregation culture (n = 9). (g) Box and whisker plot of the number of living cells per 4 mm RMF pellets. The cross indicates mean, and the thick horizontal line indicates median (n = 6). (h) Flow cytometry analysis of cell populations (MSCs, pericytes, and EPCs) within RMF pellets, SVFs, P0 ADSCs, and expanded ADSCs (n = 6). ANOVA with Dunnett’s test is used for comparison of multiple groups. *p < 0.05, **p < 0.01, ****p < 0.0001. DAPI: 4',6-diamidino-2-phenylindole.
Credit
Ru-Lin Huang, Jing Yang, Yuxin Yan, Xiangqi Liu, Xiya Yin, Chuanqi Liu, Xingran Liu, Rehanguli Aimaier, Qiumei Ji, Gen Li, Tao Zan, Kang Zhang, Qingfeng Li
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CC BY-NC-ND