Overexpression of LmTPS1 improved the resistance of transgenic tomato to biotic stress. (IMAGE)

Nanjing Agricultural University The Academy of Science

Overexpression of LmTPS1 improved the resistance of transgenic tomato to biotic stress.

Caption

Overexpression of LmTPS1 improved the resistance of transgenic tomato to biotic stress. (a) Structural diagram of LmTPS1-pCAMBIA2300. (b) Tissue-specific correlation analysis between LmTPS1 expression and β-Caryophyllene accumulation in L. megaphylla. (c) RT-qPCR verification of LmTPS1 in transgenic tomato lines (OE1-OE3). (d) Measurement of terpenoids in transgenic tomato lines and WT plants using GC–MS/MS. (e) The contents of three main terpenoids in the transgenic tomato lines. (f) The inhibitory effects of β-caryophyllene on S. aureus, E. coli, S. typhimurium, and B. subtilis. (i-h) Tomato fruits injected with β-caryophyllene presented increased resistance to biotic stress. (j) Overexpression of LmTPS1 improved the resistance of transgenic tomato to biotic stress. The leaf lesion areas were captured under 100x and 180x magnification using a super depth-of-field microscope, with arrows indicating the diseased regions on the leaf. Scale bar: 500 μm.

Credit

Horticulture Research

Usage Restrictions

Credit must be given to the creator.

License

CC BY

Disclaimer: AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert system.