Regulatory effects of ApWRKY26 and ApERF4 on ApMYB2-mediated anthocyanin biosynthesis in A. palmatum. (IMAGE)
Caption
Regulatory effects of ApWRKY26 and ApERF4 on ApMYB2-mediated anthocyanin biosynthesis in A. palmatum. a, Schematic representation of transgenic callus lines: wild-type (WT) callus, ApWRKY26-overexpressing (ApWRKY26-OE) and ApWRKY26-overexpressing with ApMYB2 co-silenced (ApWRKY26-OE + ApMYB2-RNAi). b, Anthocyanin content in WT, ApWRKY26-OE, and ApWRKY26-OE + ApMYB2-RNAi callus. c, Relative expression levels of ApMYB2 in the three callus lines. d, Schematic representation of transgenic callus lines: ApERF4 silenced (ApERF4-RNAi), ApERF4-silenced with ApMYB2 co-silenced (ApERF4-RNAi + ApMYB2-RNAi), and WT callus. e, Anthocyanin content in ApERF4-RNAi, WT, and ApERF4 RNAi + ApMYB2-RNAi callus. f, Relative expression levels of ApMYB2 in the three callus lines. g, Phenotypic comparison of A. palmatum leaves treated with water (control), antisense oligonucleotide targeting ApWRKY26 (asODN_ApWRKY26) and sense oligonucleotide (sODN_ApWRKY26). h, Relative expression levels of ApMYB2 and anthocyanin biosynthesis genes in leaves from (g). i, Phenotypic comparison of A. palmatum leaves treated with water (control), antisense oligonucleotide targeting ApERF4 (asODN_ApERF4) and sense oligonucleotide (sODN_ApERF4). j, Relative expression levels of ApMYB2 and anthocyanin biosynthesis genes in leaves from (i). Values are reported as means ± standard deviation (n = 3). Error bars correspond to SD. Lowercase letters denote significant differences between groups. Asterisks indicate pairwise comparison significance thresholds.
Credit
Horticulture Research
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