Figure | Working principle of Interferometric Image Scanning Microscopy (iISM). (IMAGE)

Light Publishing Center, Changchun Institute of Optics, Fine Mechanics And Physics, CAS

Figure | Working principle of Interferometric Image Scanning Microscopy (iISM).

Caption

Figure | Working principle of Interferometric Image Scanning Microscopy (iISM). (a) Schematic of the iISM microscope and interferometric PSF detection. (b) iISM with adaptive pixel-reassignment (APR) workflow via radial variance transform (RVT). Matrix representing the scanned interference images of a single 60 nm polystyrene nanoparticle with a 9x9 array detection. Each position corresponds to a closed off-axis virtual pinhole of 0.15 AU with respect to the central pinhole. (c) Live-cell interferometric ISM captures dynamics of intracellular organelles with enhanced contrast and resolution at about 0.5 μW incident illumination power. Scale bar 10 µm. (d) iISM close-up of (c) showing endoplasmic reticulum (ER) and vesicles (V). Scale bar 1 µm. (e) Close-up of a vesicle in (d) evaluated as C-iSCAT at open and closed pinhole and iISM after APR depicting increased resolution and contrast-to-noise ratio. Scale bars 200 nm.

Credit

Michelle Kueppers et al.

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