Figure 2. MaNAC029 interacts with MaNAC19. (IMAGE)
Caption
(a) Y2H assay for the interaction between MaNAC029 and MaNAC19. Yeast cells co-transformed with bait vector and prey vector were grown on selective medium (lacking tryptophan, leucine, histidine, and adenine), and were then stained by X-α-gal. (b) BiFC analysis of the interaction between MaNAC029 and MaNAC19 in tobacco BY-2 protoplasts. MaNAC029/19 were fused with the C terminus of YFP (YC) and the N terminus of YFP (YN), respectively, as indicated, and co-transfected into protoplasts. NLS-mCherry was used as a nuclear marker. Bars, 25 μm. (c) Co-IP assays of MaNAC029-MaNAC19 interaction. MaNAC19-GFP and MaNAC029-His, or empty GFP and MaNAC029-His, were transiently expressed in tobacco leaves and immunoprecipitated with anti-GFP antibody. Immunoprecipitated samples and input controls were detected with anti-GFP and anti-His antibodies, respectively.
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