Characterization of EV separated by distinct methods. (IMAGE)

Higher Education Press

Characterization of EV separated by distinct methods.

Caption

(a) Workflow of evaluating EV separated by ultracentrifugation (UC), EPF, and reagent precipitation (REG). (b) Representative nanoparticle tracking analysis (NTA) and transmission electron microscopy (TEM) images of UC-EVs, EPF-EVs, and REG-EVs. (c) Mean size and concentration of UC-EVs, EPF-EVs, and REG-EVs. (d) Protein concentration of the isolated EVs by UC, EPF, and REG was determined by a bicinchoninic acid (BCA) protein assay kit. (e) The particle/protein ratios of EVs by UC, EPF, and REG. UC-EVs, EPF-EVs, and REG-EVs indicated EVs isolated by UC, EPF, and REG, respectively. *P < 0.05; ****P < 0.0001; ns: no significant difference.

Credit

Yuanyuan Liu, Yanbin Guo et al.

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CC BY-NC-ND

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