Creation of grass carp without intermuscular bones through runx2b gene editing (IMAGE)

Science China Press

Creation of grass carp without intermuscular bones through runx2b gene editing

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(A) CRISPR/Cas9 targeting of the grass carp runx2b gene. (B) Injection into one-cell-stage embryos to generate F0 mutants. (C) Crossing of F0 mutants to produce F1 generation. (D) Selection of F1 individuals without IBs for paired mating to generate F2 generation.

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