Subcellular localization and interaction verification of asarf16. (IMAGE)
Caption
Subcellular localization and interaction verification of asarf16. (a) subcellular localization of asarf16. (b) y2h showed that the asarf16 interacted with aswrky31/asiaa33 by growth on sd/-l-t-h-a + x-α-gal plates. bd, pgbkt7 vector; ad, pgadt7 vector; bd-53, bd fused with p53 (positive control); bd-lam, bd fused with lamin c (negative control); ad-t, ad fused with the sv40 large t-antigen (positive control interaction partner for p53). (c) lca exhibited that asarf16 interacted with aswrky31/asiaa33, where the remaining combinations were used as negative controls. (d) bifc analysis of asarf16 and aswrky31/asiaa33, where the remaining combinations were used as negative controls.
Credit
Horticulture Research
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