Enhancer regions containing tramtrack (ttk)-binding sites (IMAGE)
Caption
CAGE can detect RNAs transcribed in opposite orientations, allowing enhancer regions to be quantitatively identified as regions where bidirectionally transcribed RNAs are present at positions separated by several hundred base pairs or more. In all panels, red (red arrows) and blue peaks (blue arrows) indicate signals on the negative strand and positive strand, respectively. Green boxes indicate predicted ttk binding sites (derived from Drosophila melanogaster motifs). Gene models are shown in blue. Corresponding enhancer regions in other bee species are displayed at the bottom of each panel. Lowercase nucleotide sequences represent repeat regions in the genome. The vertical axis represents transcription start site count data calculated by CAGE. (A) Gene structure and transcription start sites of Br-c. Arrowheads and arrows indicate individual transcription start sites. Red boxes represent enhancer regions identified in this study; regions B and C are magnified in panels (B, C). (B) An intronic enhancer region within Br-c (magnified view of region B in panel (A)). (C) An additional intronic enhancer region within Br-c (magnified view of region C in panel (A)).
Credit
Adapted from Toga et al. (2026) Insects, https://doi.org/10.3390/insects17050516. Originally published under CC BY 4.0. This version has been modified from the original.
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CC BY